Traditionally, particle analysis was performed with laser dispersion particle analysers and microscopes. In recent years, high-performance digital microscopes have been added to this list of devices due to improvements in CMOS image receiving elements, lens accuracy, lighting technology, and image processing technology.
Recent digital microscopes not only have high image quality, but also are equipped with binary image processing and templates which enables fast, accurate particle size analysis.
This section introduces examples of using the VHX Series, KEYENCE's latest 4K Digital Microscope, to solve the problems in particle size distribution and particle size analysis.

Particle Size Distribution and Size Analysis

What Are Particle Size Distribution and Particle Size Analysis Using Image Processing?

Binary image processing refers to converting an image that has gradations to black and white. The value of each pixel is compared against a set threshold; values larger than the threshold are converted to black and values smaller than the threshold are converted to white. The range over which to perform binary processing can also be specified.
Performing binary processing on an image makes it easy to extract particles from the rest of the sample, thereby making particle size analysis faster.

Binary image processing
Image pixel values
Image pixel values
Binary processed image with a threshold of 127
Binary processed image with a threshold of 127

Examples of Particle Size Distribution and Particle Size Analysis Using KEYENCE's 4K Digital Microscope

Conventional laser diffraction and laser dispersion methods cannot be used to observe actual particles.* What's more, observation with microscopes requires a high degree of proficiency, and it is difficult to reproduce the imaging conditions. Finally, the obtained data must be analysed with different software.

In particle size distribution and particle size analysis using the VHX Series 4K Digital Microscope, various measurements can be performed while observing the actual particles. Automatic area measurement using binary image processing and templates enables even inexperienced users to carry out inspection.

Laser diffraction and laser dispersion methods
* Methods in which laser emission is used for particle size distribution and particle size analysis. These measurement/analysis methods use the regularity of how the strength of the dispersed light is proportionate to the number of particles and the relationship between the size of the particles and the dispersion/diffraction pattern.

Automatic area measurement

Area measurements and counts can easily be carried out within the specified range on the target. After the binary image processing is applied (using brightness or colour data), parameters such as the area, maximum diameter, and minimum diameter are automatically calculated. Targets that are not required can be excluded, and overlapping particles can be separated and counted individually. It is also possible to perform analyses with the same conditions by using image data that was measured in the past.
Furthermore, measured values can be output to tables and histograms, which makes it possible to quantitatively determine the particle size distribution characteristics.

Automatic area measurement with binary image processing using the VHX Series 4K Digital Microscope
Observation screen
Observation screen
Particle size analysis and histogram display using binary image processing (400x)
Particle size analysis and histogram display
using binary image processing (400x)

Imaging templates

With conventional microscopes, it is extremely difficult to reproduce an image with the same conditions (lighting, shutter speed, white balance, etc.). This makes repeat inspections difficult, as two operators can get different results on the same sample.

With the VHX Series 4K Digital Microscope, past imaging settings can be reproduced just by selecting saved images from the album. The same imaging conditions as the previous observation can be used regardless of which inspector performs the observation or when the observation is performed.

Imaging setting reproduction using the VHX Series 4K Digital Microscope
Past imaging conditions can be reproduced automatically by simply selecting an image from an album.
Past imaging conditions can be reproduced automatically by simply selecting an image from an album.
Before reproduction
Before reproduction
After reproduction
After reproduction

Conventionally, calibration required the placing of a focus scale in an accurate position. However, the VHX Series enables one-push calibration; simply install a dedicated scale and perform a one-click operation. The lighting conditions, shutter speed, white balance, and other such correction values required by the lens are loaded, and motorised control of the X, Y, and Z axes is performed to automatically align the positions and the focus.

Advanced automatic zoom function and calibration function of the VHX Series 4K Digital Microscope
A. High-resolution HR lens B. Motorised revolver
  • A. High-resolution HR lens
  • B. Motorised revolver
One-click automatic calibration is possible.
One-click automatic calibration is possible.

Furthermore, the VHX Series is fully loaded with functions that reduce the load of particle size analysis such as a high-resolution HR lens and a motorised revolver that enable seamless zoom that can change the magnification between 20x and 6000x without lens replacement. All operations are easy to perform, so even people who have difficulty handling machines can quickly and accurately perform observation and analysis.

View, Capture, and Measure with Just One Device

The VHX Series high-definition 4K Digital Microscope offers excellent efficiency compared to conventional microscopes and makes it possible to eliminate human errors and ensure accurate particle size distribution and particle size analysis.
The high-definition 4K images generated by the cutting-edge optical image processing and automation techniques allow for particle size distribution and particle size analysis with simple operation, contributing to producing quick, sophisticated analysis results.
The captured or measured data can be easily output as a report with a fixed format using the report function. Sharing this data via an intranet server can be useful not only for quality assurance, but also for identifying the cause of problems as they occur.
Equipped with many other advanced functions, the VHX Series can be a powerful partner for particle size distribution and particle size analysis. For additional product info or inquiries, click the buttons below.